Effects of Various Mung Bean Decoction Doses on Hematological Profile in Anemic Wistar Rats
DOI:
https://doi.org/10.36858/jmid.v3i1.32Keywords:
mung bean decoction, anemia, hemoglobin, Wistar rats, hematological profileAbstract
Background: Anemia remains a significant global public health problem, with iron deficiency being the primary cause. Mung beans (Vigna radiata L.) are nutritionally rich plants containing iron and glycine, which are essential for hemoglobin synthesis and red blood cell formation
Purpose: This study aimed to evaluate the effects of various doses of mung bean decoction on hematological profile improvement in anemic Wistar rats induced with sodium nitrite (NaNO₂).
Methods: An experimental study was conducted from June to August 2024 involving 25 male Wistar rats divided into five groups: healthy control (aquadest), negative control (NaNO₂), and three treatment groups receiving NaNO₂ followed by mung bean decoction at doses of 0.2 ml, 0.4 ml, and 0.8 ml respectively. Blood samples were collected before and after treatment for hemoglobin level assessment. Data were analyzed using paired sample t-test and One-way ANOVA with post-hoc Tukey tests.
Results: The One-way ANOVA test showed highly significant differences in hemoglobin level changes between groups (p < 0.0001). The 0.2 ml dose group exhibited the highest hemoglobin increase from 8.12 g/dl to 17.70 g/dl (p = 0.0002), followed by the 0.4 ml dose group with an increase from 7.32 g/dl to 16.08 g/dl (p < 0.0001), and the 0.8 ml dose group with an increase from 6.40 g/dl to 14.16 g/dl (p = 0.0005).
Conclusions: Mung bean decoction effectively improved hemoglobin levels in anemic Wistar rats, with the 0.2 ml dose demonstrating the most pronounced therapeutic effect.
References
Gardner WM, Razo C, McHugh TA, Hagins H, Vilchis-Tella VM, Hennessy C, et al. Prevalence, years lived with disability, and trends in anaemia burden by severity and cause, 1990–2021: findings from the Global Burden of Disease Study 2021. Lancet Haematol [Internet]. 2023;10(9):e713–34. Available from: http://dx.doi.org/10.1016/S2352-3026(23)00160-6
WHO. Global Anaemia Estimates 2023 [Internet]. World Health Organization. 2025. Available from: https://www.who.int/data/gho
Kementerian Kesehatan RI. Survei Kesehatan Indonesia 2023 (SKI). Kemenkes. 2023. 235 p.
Riskesdas Kemenkes. Laporan Riset Kesehatan Dasar Tahun 2018 [Internet]. Badan Penelitian dan Pengembangan Kesehatan. Jakarta: Kementerian Kesehatan Republik Indonesia; 2018. 674 p. Available from: http://labdata.litbang.kemkes.go.id/images/download/laporan/RKD/2018/Laporan_Nasional_RKD2018_FINAL.pdf
Benedict RK, Pullum TW, Riese S, Milner E. Is child anemia associated with early childhood development? A cross-sectional analysis of nine Demographic and Health Surveys. PLoS One [Internet]. 2024;19(2 February):1–16. Available from: http://dx.doi.org/10.1371/journal.pone.0298967
Fitria L. Profil Hematologi Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4, 6, dan 8 Minggu. Biog J Ilm Biol. 2014;2(2):94–100.
Ascenzi P, De Simone G, Zingale GA, Coletta M. Nitrite binding to myoglobin and hemoglobin: Reactivity and structural aspects. J Inorg Biochem. 2025;265(2):1–7.
Ripley F, Park H. Methemoglobinemia Pathophysiology Toxicokinetics. 2019. 1–7 p.
Ambarwati R. Effect of Sodium Nitrite (NaNO2) to Erithrocyte and Hemoglobin Profile in White Rats ( Rattus norvegicus ). Folia Medica Indones. 2012;48(1):1–5.
Carolin BT, Suprihatin, Indirasari, Novelia S. Pemberian Sari Kacang Hijau untuk Meningkatkan Kadar Hemoglobin pada Siswi Anemia. J Qual Women’s Heal. 2021;4(1):109–14.
Mehta N, Rao P, Saini R. A review on metabolites and pharmaceutical potential of food legume crop mung bean (Vigna radiata L. Wilczek). Biotechnologia. 2021;102(4):425–35.
Yi-Shen Z, Shuai S, Fitzgerald R. Mung bean proteins and peptides: Nutritional, functional and bioactive properties. Food Nutr Res. 2018;62:1–11.
Ogun AS, Joy N V., Valentine M. Biochemistry , Heme Synthesis. Brooklyn: NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.; 2025. 1–5 p.
Sheet I, Kowalewska A. Integrating Gender and Nutrition within Agricultural Extension Services Mung Beans Nutritional Value and Recipes. 2018.
Wahyuni S. Defisiensi Besi dan Anemia Defisiensi Besi: Updated Literature Review. Galen J Kedokt dan Kesehat Mhs Malikussaleh. 2024;3(3):1.
Ndolu RA, Manafe DT, Lada CO. Pengaruh Pemberian Ekstrak Kecambah Kacang Hijau Terhadap Kadar Kolesterol Total Serum Tikus (Rattus Norvegicus) Galur Sprague Dawley Hiperkolesterolemia. Cendana Med J. 2020;20(2):214–21.
Lathifah NS. Pengaruh Pemberian Kacang Hijau Terhadap Kenaikan Kadar Hemoglobin Pada Ibu Hamil. 2018;4(3):139–44.
Krisnanda R. VITAMIN C MEMBANTU DALAM ABSORPSI ZAT BESI PADA ANEMIA DEFISIENSI BESI. J Penelit Perawat Prof. 2020;2(3):279–86.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Nabila Dwi Ayu Pratiwi, Hartalina Mufidah, Ahdiah Imroatul Muflihah
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
